A case of Wilson's disease combined with intracranial lipoma and dysplasia of the corpus callosum with review of the literature.

BACKGROUND: Wilson's disease (WD) is an inherited disorder of copper metabolism. Agenesis of the corpus callosum is the complete or partial absence of the major united fiber bundles connecting the cerebral hemispheres. Intracranial lipoma is an adipose tissue tumor resulting from an abnormal embryonic development of the central nervous system. The simultaneous occurrence of these three disorders is rare and has not been reported. This report focuses on the pathogenesis and association between the three disorders and highlights the importance of recognizing and effectively managing their coexistence. CASE PRESENTATION: The purpose of this study was to present a patient with coexisting WD, intracranial lipoma, and corpus callosum dysplasia. We reviewed a female patient hospitalized in 2023 with clinical manifestations of elevated aminotransferases and decreased ceruloplasmin, as well as genetic testing for an initial diagnosis of Wilson's disease. Subsequently, a cranial MRI showed corpus callosum dysplasia with short T1 signal changes in the cerebral falx, leading to a final diagnosis of Wilson's disease combined with intracranial lipoma and corpus callosum dysplasia. The patient's WD is currently stable after treatment with sodium dimercaptosulfonamide (DMPS) and penicillamine, and the patient's abnormal copper metabolism may promote the growth of intracranial lipoma. CONCLUSION: The pathogenesis of WD combined with intracranial lipoma and corpus callosum dysplasia is complex and clinically rare. The growth of intracranial lipomas may be associated with abnormal copper metabolism in WD. Abnormal copper metabolism affects lipid metabolism and triggers inflammatory responses. Therefore, early diagnosis and treatment are beneficial for improvement. Each new case of this rare co-morbidity is important as it allows for a better assessment and understanding of these cases' more characteristic clinical manifestations, which can help estimate the course of the disease and possible therapeutic options.

Electrochemical treatment of malachite green dye wastewater by pulse three-dimensional electrode method.

Water pollution is becoming more and more serious nowadays, and water resources are in shortage. As an environmentally friendly wastewater treatment technology without secondary pollution, the three-dimensional electrode method has received more and more attention. However, the conventional direct current (DC) three-dimensional electrode method has the disadvantages of high energy consumption and low current efficiency. Based on this, this work investigated the treatment of malachite green (MG) dye wastewater by pulse three-dimensional electrode method. The influences of pulse duty cycle, pulse period, electrolysis voltage, initial pH, aeration rate and Na2SO4 concentration on MG degradation were investigated. The results showed that under the optimal operating conditions of pulse duty cycle of 0.4, pulse period of 15 s, electrolysis voltage of 15 V, initial pH of 5, aeration rate of 0.5 L/min, Na2SO4 concentration of 0.10 mol/L, the removal rates of MG and COD reached 96.2% and 80.5%, respectively, the current efficiency reached 93.4%, and the energy consumption was 24.2 kWh/kg COD after 150 min. Compared with DC power supply mode, the MG removal rate, COD removal rate and current efficiency were enhanced, and the energy consumption was reduced by 83.9%. Moreover, the generation capacity of ·OH was increased under pulse power supply mode. Finally, a possible degradation pathway of MG in pulse power supply mode was inferred using UV-vis and GC-MS analysis. This study indicates that the pulse three-dimensional electrode method is an efficient and low-energy-consumption wastewater treatment method with stable degradation performance for MG dye wastewater.

Altered Pattern of Immunoglobulin A-Targeted Microbiota in Inflammatory Bowel Disease After Fecal Transplantation.

Adaptive immune response to the gut microbiota is one of the main drivers of inflammatory bowel disease (IBD). Under inflammatory conditions, immunoglobulin (Ig)-targeted bacteria are altered. However, changes in Ig-targeted bacteria in Asian patients with IBD with ulcerative colitis (UC) remain unclear. Furthermore, changes in IgA-targeted bacteria in patients with UC treated with fecal microbiota transplantation (FMT) are unclear. Here, we analyzed fecal samples of patients with IBD and patients with UC before and after FMT by flow cytometry. We found that the percentage of IgA/G-coated bacteria can be used to assess the severity of IBD. Besides oral pharyngeal bacteria such as Streptococcus, we hypothesized that Megamonas, Acinetobacter, and, especially, Staphylococcus might play an important role in IBD pathogenesis. Moreover, we evaluated the influence of FMT on IgA-coated bacteria in patients with UC. We found that IgA-bacterial interactions were re-established in human FMT recipients and resembled those in the healthy fecal donors. Additionally, the IgA targeting was not influenced by delivery methods: gastroscopy spraying and colonic transendoscopic enteral tubing (TET). Then, we established an acute dextran sulfate sodium (DSS)-induced mouse model to explore whether FMT intervention would impact IgA/G memory B cell in the intestine. We found that after FMT, both IgA/G memory B cell and the percentage of IgA/G-targeted bacteria were restored to normal levels in DSS mice.

Telomere is shortened in patients with irritable bowel syndrome in the Chinese population.

BACKGROUND AND AIM: Telomere shortening is an accepted indicator of aging. Many studies have investigated an association between leukocyte telomere length (LTL) and psychiatric disorders. Mental or psychological factors could be an important cause of irritable bowel syndrome (IBS). However, there are currently few research evaluating correlations between LTL and IBS. METHODS: We examined associations between LTL and IBS using quantitative polymerase chain reaction in independent cohorts, including 205 patients with IBS and 189 healthy controls. Furthermore, we examined whether mental or psychological factors, types of IBS, duration of IBS and antidepressants had an association with LTL in patients with IBS. RESULTS: Among total samples, patients with IBS presented shorter LTL when compared to healthy controls (P < 0.0001). Moreover, in subgroup analyses of patients with IBS, not only the LTL in patients with IBS caused by mental or psychological factors was shorter (P < 0.0001), but also in patients with IBS that were caused by other factors (P = 0.0082). Furthermore, LTL in patients with IBS who had taken antidepressants for more than 1 month was longer than that in patients with IBS who did not take antidepressants or took for less than 1 month (P < 0.0001). CONCLUSIONS: This is the first study to describe the relationship between LTL and IBS. This study showed significantly shorter telomeres in patients with IBS. Our findings suggest that LTL may hold the potential to serve as a predictor of IBS diagnosis.

The aberrant expressions of MACC1, ZEB1, and KLF4 in hepatocellular carcinoma and their clinical significance.

BACKGROUND AND OBJECTIVE: Metastasis-associated in colon cancer-1 (MACC1) is involved in the progression and metastasis of various cancers. Zinc finger E-box-binding homeobox 1 (ZEB1) is a key transcriptional factor of the epithelial-mesenchymal transition (EMT) that is involved in the migration and invasion of cancer cells. Kruppel-like factor 4 (KLF4) is a tumor suppressor that can inhibit tumor cell proliferation, migration, and metastasis. The purpose of this study was to investigate the expressions and clinical significance of MACC1, ZEB1, and KLF4 in hepatocellular carcinoma (HCC). METHODS: We analyzed the expressions of MACC1, ZEB1, and KLF4 in 153 HCC specimens and their corresponding control specimens. The patients' clinicopathological and follow-up data were also collected. RESULTS: The rates of positive expression of MACC1 and ZEB1 were significantly higher in the HCC specimens than in the control specimens, and their expressions were positively associated with the number of tumors, grades of differentiation, lymph node metastasis (LNM), and tumor-node-metastasis (TNM) stages. Inversely, the rate of positive expression of KLF4 was significantly lower in the HCC specimens than it was in the control specimens, and its expression was negatively correlated with the number of tumors, grades of differentiation, LNM, and TNM stages. The patients who expressed MACC1 or ZEB1 had a reduced overall survival (OS) when compared with patients not expressing these proteins. However, the patients who expressed KLF4 had an increased OS when compared with patients who did not show any KLF4 expression. A multivariate analysis indicated that the expressions of MACC1, ZEB1, and KLF4 and tumor size, LNM, as well as the TNM stages were independent, prognostic factors for HCC patients. CONCLUSIONS: Therefore, positive expressions of MACC1, ZEB1, and KLF4 should be correlated with the duration of OS in patients with HCC and considered promising prognostic biomarkers, as well as potential therapeutic targets for HCC.

Heavy metal rich stone-processing wastewater inhibits the growth and development of plants.

Large amounts of wastewater are generated from stone processing, which are toxic and cause serious environmental and health risks. To quantify the content of stone processing wastewater and estimate its effects on plant growth, we collected water samples from sewage outfall of four stone processing factories and nearby water bodies. The concentration of potential toxic metals were much higher in the wastewater than background controls. Wastewater inhibited plant primary root elongation, lateral root formation, and growth of aerial part. Seedlings treated with the effluents were unhealthy with deep purple leaves and usually died before flowering. Chlorophyll a/b contents and chloroplast number were reduced in those abnormal mesophyll cells. Transcriptional levels were decreased for chloroplast formation genes, but increased for those participated in chloroplast degradation and catabolism. Six out of nine tested senescence-associated genes were up-regulated. Furthermore, our results show that endogenous toxic metal levels indeed increased after wastewater treatment. Altogether, these results indicated that the potential toxic metals rich wastewater had significant inhibition on plant growth and led to senescence-associated program cell death, which could be helpful for the government and enterprises to understand the environmental risks and formulate reasonable wastewater emission standards for the stone processing industry.

Effects of modified sediments from a eutrophic lake in removing phosphorus and inhibiting phosphatase activity.

Phosphorus is one of the main limiting and strong influencing factors of eutrophication, and phosphorus controlling in lake is of great significance for eutrophication. To do this, sediment materials were taken from Dianchi Lake, a typically eutrophic lake, and modified by hexadecyltrimethylammonium bromide (CTAB) and ZnSO4 to remove phosphorus and inhibit alkaline phosphatase activity (APA). Results indicated that phosphorus removal efficiencies of sediments modified by CTAB (S-CTAB), ZnSO4 (S-Zn), and oxidized sediments (OS) were higher than that of the raw sediment (RS). Ability to absorb phosphorus varied, following the order S-Zn>S-CTAB>OS>RS. Sorption was influenced by ionic strength, with the former decreasing with the increase of the latter. Freundlich model well described the sorption isotherm, with an R2 ranging from 0.9168 to 0.9958. Furthermore, compared with the raw sediments, the maximum phosphorus sorption capacities of S-Zn and S-CTAB increased by 12.2% and 124.5%, respectively. Results of desorption studies suggest that the desorption rate of S-Zn was from 3.88 to 13.76%, lower than that of other sediment materials. APA was inhibited by S-CTAB and S-Zn at the same time, with inhibition rates from 29.6% and 61.0% when the concentrations of S-CTAB and S-Zn were 10 nmol L-1 and 0.2 nmol L-1, respectively. This study provides new insights into phosphorus removal and phosphatase activity inhibition in water treatment.

Hydrogen-Bonding Network Promoted [3+2] Cycloaddition: Asymmetric Catalytic Construction of Spiro-pseudoindoxyl Derivatives.

The enantioselective construction of a spirocyclic quaternary stereogenic carbon center at the C2 position of indole has long been an elusive problem in organic synthesis. Herein, by employing a rationally designed hydrogen-bonding network activation strategy, for the first time, 2,2'-pyrrolidinyl-spirooxindole, which is a valuable and prevalent indole alkaloid scaffold, was directly obtained through a catalytic asymmetric [3+2] cycloaddition reaction with high yields and excellent stereoselectivities.